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Nucleic Acids Res. 2010 Jul;38(12):4154-62. doi: 10.1093/nar/gkq133. Epub 2010 Mar 4.

The crystal structure of unmodified tRNAPhe from Escherichia coli.

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1
York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, North Yorkshire, YO10 5YW, UK.

Abstract

Post-transcriptional nucleoside modifications fine-tune the biophysical and biochemical properties of transfer RNA (tRNA) so that it is optimized for participation in cellular processes. Here we report the crystal structure of unmodified tRNA(Phe) from Escherichia coli at a resolution of 3 A. We show that in the absence of modifications the overall fold of the tRNA is essentially the same as that of mature tRNA. However, there are a number of significant structural differences, such as rearrangements in a triplet base pair and a widened angle between the acceptor and anticodon stems. Contrary to previous observations, the anticodon adopts the same conformation as seen in mature tRNA.

PMID:
20203084
PMCID:
PMC2896525
DOI:
10.1093/nar/gkq133
[Indexed for MEDLINE]
Free PMC Article
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