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Am J Hematol. 2010 Apr;85(4):234-7. doi: 10.1002/ajh.21642.

A flow cytometry-based assay for measuring invasion of red blood cells by Plasmodium falciparum.

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  • 1Harvard School of Public Health, Boston, Massachusetts, USA.

Abstract

Variability in the ability of the malaria parasite Plasmodium falciparum to invade human erythrocytes is postulated to be an important determinant of disease severity. Both the parasite multiplication rate and erythrocyte selectivity are important parameters that underlie such variable invasion. We have established a flow cytometry-based method for simultaneously calculating both the parasitemia and the number of multiply-infected erythrocytes. Staining with the DNA-specific dye SYBR Green I allows quantitation of parasite invasion at the ring stage of parasite development. We discuss in vitro and in vivo applications and limitations of this method in relation to the study of parasite invasion.

PMID:
20196166
PMCID:
PMC3089760
DOI:
10.1002/ajh.21642
[PubMed - indexed for MEDLINE]
Free PMC Article
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