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Mol Plant Microbe Interact. 2010 Apr;23(4):355-365. doi: 10.1094/MPMI-23-4-0355.

Role of the Sinorhizobium meliloti global regulator Hfq in gene regulation and symbiosis.

Author information

1
Soil and Water Science Department, Cancer and Genetics Research Complex, Room 330E, University of Florida-Institute of Food and Agricultural Sciences, Gainesville 32610, U.S.A.
2
Department of Biology, Stanford University, Stanford, CA 94305-5020, U.S.A.
#
Contributed equally

Abstract

The RNA-binding protein Hfq is a global regulator which controls diverse cellular processes in bacteria. To begin understanding the role of Hfq in the Sinorhizobium meliloti-Medicago truncatula nitrogen-fixing symbiosis, we defined free-living and symbiotic phenotypes of an hfq mutant. Over 500 transcripts were differentially accumulated in the hfq mutant of S. meliloti Rm1021 when grown in a shaking culture. Consistent with transcriptome-wide changes, the hfq mutant displayed dramatic alterations in metabolism of nitrogen-containing compounds, even though its carbon source utilization profiles were nearly identical to the wild type. The hfq mutant had reduced motility and was impaired for growth at alkaline pH. A deletion of hfq resulted in a reduced symbiotic efficiency, although the mutant was still able to initiate nodule development and differentiate into bacteroids.

PMID:
20192823
PMCID:
PMC4827774
DOI:
10.1094/MPMI-23-4-0355
[Indexed for MEDLINE]
Free PMC Article

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