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Annu Rev Plant Biol. 2010;61:373-93. doi: 10.1146/annurev-arplant-042809-112143.

High-throughput characterization of plant gene functions by using gain-of-function technology.

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1
Plant Functional Genomics Research Team, RIKEN Plant Science Center, Tsurumi-ku, Yokohama, Japan. youichi@psc.riken.jp

Abstract

Gain-of-function approaches have been used as an alternative or complementary method to loss-of-function approaches as well as to confer new functions to plants. Gain-of-function is achieved by increasing gene expression levels through the random activation of endogenous genes by transcriptional enhancers or the expression of individual transgenes by transformation. The advantages of gain-of-function approaches compared to loss-of-function approaches for the characterization of gene functions include the abilities to (a) analyze individual gene family members, (b) characterize the function of genes from nonmodel plants using a heterologous expression system, and (c) identify genes that confer stress tolerance to plants that result from the introduction of transgenes. In this review, we describe the current status of gain-of-function mutagenesis and provide several examples of how gene functions have been characterized via high-throughput screening using gain-of-function technology.

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