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J Mol Biol. 2010 Apr 16;397(5):1144-55. doi: 10.1016/j.jmb.2010.02.029. Epub 2010 Feb 23.

Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid.

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  • 1Department of Forensic Medicine and Tampere University Hospital, FI-33014 University of Tampere, Finland.

Abstract

We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA in such molecules is highly dependent on the preparative method used. These findings strongly support the strand-coupled model of mitochondrial DNA replication involving RNA incorporation throughout the lagging strand.

PMID:
20184890
PMCID:
PMC2857715
DOI:
10.1016/j.jmb.2010.02.029
[PubMed - indexed for MEDLINE]
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