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RNA. 2010 Apr;16(4):817-27. doi: 10.1261/rna.1712910. Epub 2010 Feb 24.

LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication.

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  • 1Department of Experimental and Health Sciences, Universitat Pompeu Fabra, 08003 Barcelona, Spain.

Abstract

LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3'-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions.

PMID:
20181739
PMCID:
PMC2844628
DOI:
10.1261/rna.1712910
[PubMed - indexed for MEDLINE]
Free PMC Article
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