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Allergol Int. 2010 Jun;59(2):161-6. doi: 10.2332/allergolint.09-OA-0103. Epub 2010 Feb 25.

Curdlan induces DC-mediated Th17 polarization via Jagged1 activation in human dendritic cells.

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  • 1Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama, Japan.

Abstract

BACKGROUND:

Th17-inducing activity is carried by certain polysaccharides such as beta-glucan derived from Candia albicans. Our previous studies have shown that Th1- and Th2-inducing activities can be qualitatively evaluated by the expression patterns of Notch ligand isoforms, using human monocyte-derived dendritic cells (Mo-DCs) and some leukemic cell lines such as THP-1. The association of Th17-inducing activities with Notch ligand expression patterns has been unclear.

METHODS:

Mo-DCs from healthy volunteers were co-cultured with HLA-DR-nonshared allogeneic CD4+ naïve T cells to induce a mixed lymphocyte reaction, in the presence of adjuvants, such as curdlan. Culture supernatants were assayed for IFNgamma, IL-5 and IL-17 by an enzyme-linked immunosorbent assay (ELISA). Notch ligand expression on Mo-DCs and THP-1 cells was evaluated by using RT-PCR.

RESULTS:

The present study shows that curdlan, one of the beta-glucans, has the ability to induce DC-mediated Th17 differentiation. It is also interesting to note that Jagged1 mRNA in Mo-DCs and THP-1 cells is up-regulated by curdlan. Furthermore, polyclonal anti-Jagged1 antibody inhibited such DC-mediated Th17 differentiation.

CONCLUSIONS:

This study suggests that curdlan induces human DC-mediated Th17 polarization via Jagged1 activation in DCs.

PMID:
20179419
DOI:
10.2332/allergolint.09-OA-0103
[PubMed - indexed for MEDLINE]
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