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In Vitro Cell Dev Biol Anim. 2010 Apr;46(3-4):386-94. doi: 10.1007/s11626-010-9291-5. Epub 2010 Feb 23.

Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines.

Author information

1
North West Embryonic Stem Cell Centre, Faculty of Life Sciences, University of Manchester, Ground Floor CTF Building, Oxford Road, Manchester M13 3PT, UK. mavi.camarasa@caubet-cimera.es

Abstract

We report here the derivation of two new human embryonic stem cell lines, Man-1 and Man-2, and their full characterization as novel pluripotent stem cell lines. Man-1 was derived from an embryo surplus to requirement from routine IVF, while Man-2 was obtained from an oocyte classified as failed to fertilise and subsequently chemically activated. We report the characterisation of pluripotency and the differentiation potential of these lines. Work is in progress to establish novel methods of stem cell derivation and culture, which will avoid the use of xenobiotics and be relevant to clinical production of human embryonic stem cell lines. Both newly derived human embryonic stem cell lines will be available for the research community from the UK Stem Cell Bank (http://www.ukstemcellbank.org.uk).

PMID:
20177994
DOI:
10.1007/s11626-010-9291-5
[Indexed for MEDLINE]

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