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BMC Plant Biol. 2010 Feb 22;10:33. doi: 10.1186/1471-2229-10-33.

STIL, a peculiar molecule from styles, specifically dephosphorylates the pollen receptor kinase LePRK2 and stimulates pollen tube growth in vitro.

Author information

1
Instituto de Ingeniería Genética y Biología Molecular, CONICET, Buenos Aires, Argentina.

Abstract

BACKGROUND:

LePRK1 and LePRK2 are two pollen receptor kinases localized to the plasma membrane, where they are present in a high molecular weight complex (LePRK complex). LePRK2 is phosphorylated in mature and germinated pollen, but is dephosphorylated when pollen membranes are incubated with tomato or tobacco style extracts.

RESULTS:

Here we show that LePRK2 dephosphorylation is mediated by a heat-, acid-, base-, DTT- and protease-resistant component from tobacco styles. Using LePRK2 phosphorylation as a tracking assay for purification, style exudates were subjected to chloroform extraction, anionic exchange, and C18 reverse-phase chromatography columns. We finally obtained a single ~3,550 Da compound (as determined by UV-MALDI-TOF MS) that we named STIL (for Style Interactor for LePRKs). STIL increased pollen tube lengths of in vitro germinated pollen in a dose-dependent manner.

CONCLUSION:

We propose that the LePRK complex perceives STIL, resulting in LePRK2 dephosphorylation and an increase in pollen tube growth.

PMID:
20175921
PMCID:
PMC2844069
DOI:
10.1186/1471-2229-10-33
[Indexed for MEDLINE]
Free PMC Article

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