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Opt Express. 2010 Feb 1;18(3):3023-34. doi: 10.1364/OE.18.003023.

Depth of field multiplexing in microscopy.

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  • 1Innsbruck Medical University, Division for Biomedical Physics, Müllerstrasse 44, 6020 Innsbruck, Austria.


We demonstrate "depth of field multiplexing" by a high resolution spatial light modulator (SLM) in a Fourier plane in the imaging path of a standard microscope. This approach provides simultaneous imaging of different focal planes in a sample with only a single camera exposure. The phase mask on the SLM corresponds to a set of superposed multi-focal off-axis Fresnel lenses, which sharply image different focal planes of the object to non-overlapping adjacent sections of the camera chip. Depth of field multiplexing allows to record motion in a three dimensional sample volume in real-time, which is exemplarily demonstrated for cytoplasmic streaming in plant cells and rapidly swimming protozoa.

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