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Nucleic Acids Res. 1991 Feb 25;19(4):727-31.

Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors.

Author information

1
Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, FRG.

Abstract

For the isolation of single stranded plasmid DNA, various E. coli and E. coli-Streptomyces shuttle plasmids were equipped with the phage f1 replication origin. The transformation of some representative Streptomyces species with plasmid vectors occurred irrespective of whether single or double stranded DNA was used. In contrast, the transformation of Streptomyces was 10 to 100 times more efficient when an integration vector was in the single stranded form as opposed to the double stranded form. Streptomyces viridochromogenes was transformed by single stranded DNA integration vectors in order to replace the pat by the tsr gene and generate mutants unable to synthesize phosphinothricin-tripeptide (PTT).

PMID:
2017360
PMCID:
PMC333703
DOI:
10.1093/nar/19.4.727
[Indexed for MEDLINE]
Free PMC Article

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