Format

Send to

Choose Destination
See comment in PubMed Commons below
Fertil Steril. 2010 Nov;94(6):2088-95. doi: 10.1016/j.fertnstert.2009.12.065. Epub 2010 Feb 19.

Prospective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification.

Author information

  • 1Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, Michigan, USA.

Abstract

OBJECTIVE:

To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy.

DESIGN:

Prospective randomized.

SETTING:

Academically affiliated, private fertility center.

PATIENT(S):

Consenting patients with concerns about embryo cryopreservation and more than nine mature oocytes at retrieval were randomized to slow-rate freezing or vitrification of supernumerary (more than nine) oocytes.

INTERVENTION(S):

Oocytes were frozen or vitrified, and upon request oocytes were thawed or warmed, respectively.

MAIN OUTCOME MEASURE(S):

Oocyte survival, fertilization, embryo development, and clinical pregnancy.

RESULT(S):

Patient use has resulted in 30 thaws and 48 warmings. Women's age at time of cryopreservation was similar. Oocyte survival was significantly higher following vitrification/warming (81%) compared with freezing/thawing (67%). Fertilization was more successful in oocytes vitrified/warmed compared with frozen/thawed. Fertilized oocytes from vitrification/warming had significantly better cleavage rates (84%) compared with freezing/thawing (71%) and resulted in embryos with significantly better morphology. Although similar numbers of embryos were transferred, embryos resulting from vitrified oocytes had significantly enhanced clinical (38%) pregnancy rates compared with embryos resulting from frozen oocyte (13%). Miscarriage and/or spontaneous abortion rates were similar.

CONCLUSION(S):

Our results suggest that vitrification/warming is currently the most efficient means of oocyte cryopreservation in relation to subsequent success in establishing pregnancy.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center