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J Proteomics. 2010 May 7;73(7):1404-9. doi: 10.1016/j.jprot.2010.02.008. Epub 2010 Feb 16.

Identification of Candida albicans exposed surface proteins in vivo by a rapid proteomic approach.

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Unidad de Proteómica, Universidad Complutense de Madrid-Parque Científico de Madrid (UCM-PCM), Spain.


We have set up a fast and easy methodology to identify cell-surface proteins in live yeasts. A non-gel proteomic approach was based on a short period of trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS. Candida albicans was used as a model organism and proteins involved in cell wall organization, cell rescue, defense, virulence, transport, protein fate and metabolism were identified. This strategy is a powerful tool to study host-pathogen interactions and to look for potential vaccine candidates and drug targets.

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