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J AOAC Int. 2009 Nov-Dec;92(6):1763-72.

Estimation of the POD function and the LOD of a qualitative microbiological measurement method.

Author information

1
Bundesanstalt für Materialforschung und -prüfung, Unter den Eichen 87, D-12200 Berlin, Germany. wilrich@wiwiss.fu-berlin.de

Abstract

Qualitative microbiological measurement methods in which the measurement results are either 0 (microorganism not detected) or 1 (microorganism detected) are discussed. The performance of such a measurement method is described by its probability of detection as a function of the contamination (CFU/g or CFU/mL) of the test material, or by the LOD(p), i.e., the contamination that is detected (measurement result 1) with a specified probability p. A complementary log-log model was used to statistically estimate these performance characteristics. An intralaboratory experiment for the detection of Listeria monocytogenes in various food matrixes illustrates the method. The estimate of LOD50% is compared with the Spearman-Kaerber method.

PMID:
20166595
[Indexed for MEDLINE]

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