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Mycopathologia. 2010 Jun;169(6):445-9. doi: 10.1007/s11046-010-9284-7. Epub 2010 Feb 18.

An improved protocol for the preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter papers.

Author information

1
Mycology Reference Laboratory, Health Protection Agency, South-West Regional Laboratory, Bristol, UK. Andy.Borman@uhBristol.nhs.uk

Abstract

Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology.

PMID:
20165922
DOI:
10.1007/s11046-010-9284-7
[Indexed for MEDLINE]

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