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Anal Chem. 2010 Mar 15;82(6):2192-203. doi: 10.1021/ac9024889.

Single-molecule spectroscopy and imaging of biomolecules in living cells.

Author information

1
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.

Abstract

The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a single molecule were completed over two decades ago. Single-molecule spectroscopy has developed into a field that includes a wealth of experiments at room temperature and inside living cells. The fast growth of single-molecule biophysics has resulted from its benefits in probing heterogeneous populations, one molecule at a time, as well as from advances in microscopes and detectors. This Perspective summarizes the field of live-cell imaging of single biomolecules.

PMID:
20163145
PMCID:
PMC2838489
DOI:
10.1021/ac9024889
[Indexed for MEDLINE]
Free PMC Article

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