Fetal calf serum-free suspension culture of Chinese hamster ovary cells employing fish serum

Masashi Fujiwara; Yu Aizu; Itaru Shioya; Mutsumi Takagi

doi:10.1016/j.jbiosc.2009.08.481

Fetal calf serum-free suspension culture of Chinese hamster ovary cells employing fish serum

J Biosci Bioeng. 2010 Mar;109(3):307-9. doi: 10.1016/j.jbiosc.2009.08.481. Epub 2009 Sep 16.

Authors

Masashi Fujiwara¹, Yu Aizu, Itaru Shioya, Mutsumi Takagi

Affiliation

¹ Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, North 13, West 8, Kita-ku, Sapporo 060-8628, Japan.

PMID: 20159583
DOI: 10.1016/j.jbiosc.2009.08.481

Abstract

The effects of heat treatment and concentration of fish serum (FS) on cell growth in a suspension culture of recombinant Chinese hamster ovary (CHO) 1-15(500) (ATCC CRL-9606) cells were investigated. An increase in FS concentration from 1% to 4% markedly increased cell density. On the other hand, heat treatment of FS showed nearly no effect on cell density.

MeSH terms

Animals
CHO Cells
Cattle / blood*
Cell Culture Techniques / methods*
Cricetinae
Cricetulus
Culture Media, Serum-Free
Fishes / blood*
Serum / metabolism*

Substances

Culture Media, Serum-Free