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Nat Methods. 2010 Mar;7(3):206-8. doi: 10.1038/nmeth.1428. Epub 2010 Feb 14.

Organelle-specific, rapid induction of molecular activities and membrane tethering.

Author information

1
Department of Cell Biology, Center for Cell Dynamics, School of Medicine, Baltimore, Maryland, USA.

Abstract

Using new chemically inducible dimerization probes, we generated a system to rapidly target proteins to individual intracellular organelles. Using this system, we activated Ras GTPase at distinct intracellular locations and induced tethering of membranes from two organelles, endoplasmic reticulum and mitochondria. Innovative techniques to rapidly perturb molecular activities and organelle-organelle communications at precise locations and timing will provide powerful strategies to dissect spatiotemporally complex biological processes.

PMID:
20154678
PMCID:
PMC2860863
DOI:
10.1038/nmeth.1428
[Indexed for MEDLINE]
Free PMC Article

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