Troubleshooting methods for APP processing in vitro

J Pharmacol Toxicol Methods. 2010 Mar-Apr;61(2):86-91. doi: 10.1016/j.vascn.2010.02.003. Epub 2010 Feb 12.

Abstract

Introduction: The amyloid hypothesis states that Abeta is the main trigger for Alzheimer's disease. This report is focused in the study of the processing of the Amyloid Precursor Protein (APP) as a procedure to investigate the molecular mechanisms that may result in changes in the levels of Abeta.

Methods: Here we analyse different methodologies for Abeta determination, soluble APP, APP-Carboxy terminus fragments (CTFs) and enzymes for synthesis (secretases) and degradation of Abeta. In addition the advantages and disadvantages of different methodologies are discussed.

Discussion: The potential value of these procedures is described in the context of the function of APP and the different fragments derived from its cleavage.

Publication types

  • Review

MeSH terms

  • Alzheimer Disease / etiology*
  • Amyloid Precursor Protein Secretases / analysis
  • Amyloid Precursor Protein Secretases / metabolism
  • Amyloid beta-Protein Precursor / analysis
  • Amyloid beta-Protein Precursor / metabolism
  • Amyloid beta-Protein Precursor / toxicity*
  • Animals
  • Biotinylation
  • Blotting, Western
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Microscopy, Confocal
  • Peptide Fragments / analysis
  • Spectrum Analysis
  • Subcellular Fractions / chemistry
  • Subcellular Fractions / metabolism
  • Toxicity Tests / methods*
  • Toxicity Tests / standards

Substances

  • Amyloid beta-Protein Precursor
  • Peptide Fragments
  • Amyloid Precursor Protein Secretases