Format

Send to

Choose Destination
J Biol Chem. 2010 Apr 9;285(15):11039-44. doi: 10.1074/jbc.R109.091306. Epub 2010 Feb 10.

Beyond the canonical 20 amino acids: expanding the genetic lexicon.

Author information

1
Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037, USA.

Abstract

The ability to genetically encode unnatural amino acids beyond the common 20 has allowed unprecedented control over the chemical structures of recombinantly expressed proteins. Orthogonal aminoacyl-tRNA synthetase/tRNA pairs have been used together with nonsense, rare, or 4-bp codons to incorporate >50 unnatural amino acids into proteins in Escherichia coli, Saccharomyces cerevisiae, Pichia pastoris, and mammalian cell lines. This has allowed the expression of proteins containing amino acids with novel side chains, including fluorophores, post-translational modifications, metal ion chelators, photocaged and photocross-linking moieties, uniquely reactive functional groups, and NMR, IR, and x-ray crystallographic probes.

PMID:
20147747
PMCID:
PMC2856976
DOI:
10.1074/jbc.R109.091306
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center