Abstract
Human adenoviruses (AdVs) typically cause mild illnesses in otherwise healthy hosts. We investigated a pediatric outbreak of acute respiratory infection with fatal outcomes that occurred in Lisbon, Portugal, in 2004. Biological specimens were collected from 83 children attending two nurseries, a kinesiotherapy clinic, and the household of a nanny. Adenovirus infection was confirmed in 48 children by PCR and virus isolation. Most (96%) isolates were classified as being of subspecies B1. Phylogenetic analysis of fiber and hexon gene sequences revealed that most infants were infected with AdV serotype 3 (AdV3) strains. Infants attending one nursery harbored a new recombinant strain containing an AdV serotype 7 hexon and serotype 3 fiber (AdV7/3). Both the AdV3 and the AdV7/3 strains caused fatal infections. Two different serotype 3 strains were circulating in Lisbon in 2004, and the new AdV7/3 recombinant type originated from only one of those strains. These results demonstrate that recombination leads to the emergence of new adenovirus strains with epidemic and lethal potential.
MeSH terms
-
Adenoviridae Infections / epidemiology*
-
Adenoviridae Infections / virology
-
Adenoviruses, Human / classification
-
Adenoviruses, Human / genetics
-
Adenoviruses, Human / immunology
-
Adenoviruses, Human / isolation & purification*
-
Capsid Proteins / genetics
-
Child, Preschool
-
Cluster Analysis
-
DNA, Viral / chemistry
-
DNA, Viral / genetics
-
Disease Outbreaks*
-
Genotype
-
Humans
-
Infant
-
Infant, Newborn
-
Molecular Epidemiology
-
Molecular Sequence Data
-
Phylogeny
-
Portugal / epidemiology
-
Recombination, Genetic
-
Respiratory Tract Infections / epidemiology*
-
Respiratory Tract Infections / virology
-
Sequence Analysis, DNA
Substances
-
Capsid Proteins
-
DNA, Viral
-
hexon capsid protein, Adenovirus
Associated data
-
GENBANK/GQ922009
-
GENBANK/GQ922010
-
GENBANK/GQ922011
-
GENBANK/GQ922012
-
GENBANK/GQ922013
-
GENBANK/GQ922014
-
GENBANK/GQ922015
-
GENBANK/GQ922016
-
GENBANK/GQ922017
-
GENBANK/GQ922018
-
GENBANK/GQ922019
-
GENBANK/GQ922020
-
GENBANK/GQ922021
-
GENBANK/GQ922022
-
GENBANK/GQ922023
-
GENBANK/GQ922024
-
GENBANK/GQ922025
-
GENBANK/GQ922026
-
GENBANK/GQ922027
-
GENBANK/GQ922028
-
GENBANK/GQ922029
-
GENBANK/GQ922030
-
GENBANK/GQ922031
-
GENBANK/GQ922032
-
GENBANK/GQ922033
-
GENBANK/GQ922034
-
GENBANK/GQ922035
-
GENBANK/GQ922036
-
GENBANK/GQ922037
-
GENBANK/GQ922038
-
GENBANK/GQ922039
-
GENBANK/GQ922040
-
GENBANK/GQ922041
-
GENBANK/GQ922042
-
GENBANK/GQ922043
-
GENBANK/GQ922044
-
GENBANK/GQ922045
-
GENBANK/GQ922046
-
GENBANK/GQ922047
-
GENBANK/GQ922048
-
GENBANK/GQ922049
-
GENBANK/GQ922050
-
GENBANK/GQ922051