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Chirality. 2010 Aug;22(8):726-33. doi: 10.1002/chir.20824.

Determination of catechin diastereomers from the leaves of Byrsonima species using chiral HPLC-PAD-CD.

Author information

1
Institute of Chemistry, São Paulo State University, Unesp, Francisco Degni Street, Araraquara-SP, Brazil.

Abstract

When catechins are found in plant extracts, they are almost always identified as catechin and/or epicatechin probably due to stereoselectivity of the enzymes involved in the biosynthesis of these substances. However, the lack of reports regarding to ent-catechin as well as ent-epicatechin does not necessarily mean that these compounds have not been produced. In fact, most of the previous reports used chromatographic conditions not suitable for such separation. This article describes a simple and reliable analytical HPLC-PAD-CD method for simultaneous determination of catechin diastereomers both in infusions and extracts from the leaves of Byrsonima species. The direct separation of catechin, ent-catechin, epicatechin, and ent-epicatechin was obtained in normal phase by HPLC-PAD-CD using Chiralcel OD-H as chiral stationary phase and n-hexane/ethanol with 0.1% of TFA as mobile phase.

PMID:
20143413
DOI:
10.1002/chir.20824
[Indexed for MEDLINE]

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