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Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3458-62. doi: 10.1073/pnas.1000398107. Epub 2010 Feb 8.

Mechanism of chromatin remodeling.

Author information

1
Department of Structural Biology, Stanford School of Medicine, Stanford, CA 94305, USA. lorch@stanford.edu

Abstract

Results from biochemical and structural studies of the RSC chromatin-remodeling complex prompt a proposal for the remodeling mechanism: RSC binding to the nucleosome releases the DNA from the histone surface and initiates DNA translocation (through one or a small number of DNA base pairs); ATP binding completes translocation, and ATP hydrolysis resets the system. Binding energy thus plays a central role in the remodeling process. RSC may disrupt histone-DNA contacts by affecting histone octamer conformation and through extensive interaction with the DNA. Bulging of the DNA from the octamer surface is possible, and twisting is unavoidable, but neither is the basis of remodeling.

PMID:
20142505
PMCID:
PMC2817641
DOI:
10.1073/pnas.1000398107
[Indexed for MEDLINE]
Free PMC Article

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