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Chem Biol. 2010 Jan 29;17(1):38-45. doi: 10.1016/j.chembiol.2009.12.015.

Seamless bead to microarray screening: rapid identification of the highest affinity protein ligands from large combinatorial libraries.

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1
Departments of Internal Medicine and Molecular Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9185, USA.

Abstract

Several approaches have been developed for screening combinatorial libraries or collections of synthetic molecules for agonists or antagonists of protein function, each with its own advantages and limitations. In this report, we describe an experimental platform that seamlessly couples massively parallel bead-based screening of one-bead one-compound combinatorial libraries with microarray-based quantitative comparisons of the binding affinities of the many hits isolated from the bead library. Combined with other technical improvements, this technique allows the rapid identification of the best protein ligands in combinatorial libraries containing millions of compounds without the need for labor-intensive resynthesis of the hits.

PMID:
20142039
PMCID:
PMC2905650
DOI:
10.1016/j.chembiol.2009.12.015
[Indexed for MEDLINE]
Free PMC Article
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