Format

Send to

Choose Destination
See comment in PubMed Commons below
J Am Chem Soc. 2010 Mar 3;132(8):2585-90. doi: 10.1021/ja9074562.

Live-cell one- and two-photon uncaging of a far-red emitting acridinone fluorophore.

Author information

1
Laboratoire de Conception et Application de Molécules Bioactives, UMR 7199 CNRS, Faculté de Pharmacie, Université de Strasbourg, 74 Route du Rhin 67401 Illkirch Cédex, France.

Abstract

Total synthesis and photophysical properties of PENB-DDAO, a photoactivatable 1,3-dichloro-9,9-dimethyl-9H-acridin-2(7)-one (DDAO) derivative of a far-red emitting fluorophore, are described. The photoremovable group of the DDAO phenolic function comprises a donor/acceptor biphenyl platform which allows an efficient (> or = 95%) and rapid (< 15 micros time-range) release of the fluorescent signal and displays remarkable two-photon uncaging cross sections (delta(a) x Phi(u) = 3.7 GM at 740 nm). PENB-DDAO is cell permeable as demonstrated by the triggering of cytoplasmic red fluorescent signal in HeLa cells after one-photon irradiation (lambda(exc) around 360 nm) or by the generation of a red fluorescent signal in a delineated area of a single cell after two-photon photoactivation (lambda(exc) = 770 nm).

PMID:
20141131
DOI:
10.1021/ja9074562
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Support Center