Format

Send to

Choose Destination
Pflugers Arch. 2010 Mar;459(4):557-67. doi: 10.1007/s00424-009-0770-z. Epub 2010 Feb 6.

Genetically modified mice-successes and failures of a widely used technology.

Author information

1
Institute of Physiology, University of Regensburg, Universitätsstr. 31, 93040 Regensburg, Germany. hayo@castrop.com

Abstract

Genetically modified mice, created by random integration of a transgene into the genome or by targeted mutation of a specific gene, have proven to be extremely powerful tools for studying gene function in vivo. In this article, we give (1) a short overview of the traditional methods in mouse transgenesis and (2) a discussion of the problems with these methods, (3) more recent methods that were developed to overcome these problems, and (4) an outlook on future directions in gene targeting.

PMID:
20140450
DOI:
10.1007/s00424-009-0770-z
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center