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Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):3163-8. doi: 10.1073/pnas.0911979107. Epub 2010 Jan 28.

Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein.

Author information

1
Mikrobiologie, Fakultät für Biologie, Universität Freiburg, 79104 Freiburg, Germany. joel.defeusoufo@biologie.uni-freiburg.de

Abstract

We show that translation initiation factor EF-Tu plays a second important role in cell shape maintenance in the bacterium Bacillus subtilis. EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal element setting up rod cell shape. The localization of MreB and of EF-Tu is interdependent, but in contrast to the dynamic MreB filaments, EF-Tu structures are more static and may serve as tracks for MreB filaments. In agreement with this idea, EF-Tu and MreB interact in vivo and in vitro. Lowering of the EF-Tu levels had a minor effect on translation but a strong effect on cell shape and on the localization of MreB, and blocking of the function of EF-Tu in translation did not interfere with the localization of MreB, showing that, directly or indirectly, EF-Tu affects the cytoskeletal MreB structure and thus serves two important functions in a bacterium.

PMID:
20133608
PMCID:
PMC2840354
DOI:
10.1073/pnas.0911979107
[Indexed for MEDLINE]
Free PMC Article

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