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Biomacromolecules. 2010 Mar 8;11(3):806-14. doi: 10.1021/bm901429q.

Design of double stimuli-responsive polyelectrolyte microcontainers for protein soft encapsulation.

Author information

1
DSV/iBEB/SBTN/LEPC, ICSM, UMR 5257 CEACNRS-UM2-ENSCM, CEA Marcoule, BP 17171, 30207 Bagnols-sur-Ceze cedex, France.

Abstract

We have designed new double stimuli-responsive polyelectrolyte microcapsules to be useable under physiological conditions to handle biomacromolecules while avoiding the risk of denaturation. They are made of poly(4-vinylpyridine hydrochloride) (PVP) and poly(sodium styrene sulfonate) (PSS). The microcontainers are sensitive to temperature variation, as they irreversibly shrink under heating. In addition, the capsules reversibly swell at pH > 6, making it possible to encapsulate human serum proteins by diffusion through the polymer membrane. Encapsulation efficiency is quantified by fluorescence techniques.

PMID:
20131894
DOI:
10.1021/bm901429q
[Indexed for MEDLINE]

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