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Biotechnol Lett. 2010 May;32(5):661-7. doi: 10.1007/s10529-010-0209-9. Epub 2010 Feb 4.

Genetic changes that increase 5-hydroxymethyl furfural resistance in ethanol-producing Escherichia coli LY180.

Author information

1
Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA.

Abstract

The ability of a biocatalyst to tolerate furan inhibitors present in hemicellulose hydrolysates is important for the production of renewable chemicals. This study shows EMFR9, a furfural-tolerant mutant of ethanologenic E. coli LY180, has also acquired tolerance to 5-hydroxymethyl furfural (5-HMF). The mechanism of action of 5-HMF and furfural appear similar. Furan tolerance results primarily from lower expression of yqhD and dkgA, two furan reductases with a low K(m) for NADPH. Furan tolerance was also increased by adding plasmids encoding a NADPH/NADH transhydrogenase (pntAB). Together, these results support the hypothesis that the NADPH-dependent reduction of furans by YqhD and DkgA inhibits growth by competing with biosynthesis for this limiting cofactor.

PMID:
20131081
PMCID:
PMC2852031
DOI:
10.1007/s10529-010-0209-9
[Indexed for MEDLINE]
Free PMC Article

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