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Nat Struct Mol Biol. 2010 Feb;17(2):244-7. doi: 10.1038/nsmb.1734. Epub 2010 Jan 31.

Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae.

Author information

1
Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio, USA.

Abstract

Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5' end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.

PMID:
20118937
PMCID:
PMC2840703
DOI:
10.1038/nsmb.1734
[Indexed for MEDLINE]
Free PMC Article

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