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Fertil Steril. 2010 Nov;94(6):2141-6. doi: 10.1016/j.fertnstert.2009.12.030. Epub 2010 Feb 1.

Semen characteristics and sperm DNA fragmentation in infertile men with low and high levels of seminal reactive oxygen species.

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  • 1Center for Reproductive Medicine, Glickman Urological & Kidney Institute, Cleveland Clinic, Cleveland, Ohio 44195, USA.

Abstract

OBJECTIVE:

To examine sperm motility, total antioxidant level (TAC), DNA fragmentation, and medical history in infertile men with high seminal high reactive oxygen species (ROS).

DESIGN:

Prospective study.

SETTING(S):

Tertiary care hospital.

PATIENT(S):

Infertile men (n=101).

INTERVENTION(S):

Group I (n=57) included men with seminal ROS (<250 relative light units/sec/×10(6) sperm) while group II (n=44) included men with seminal ROS levels (≥250 relative light units/sec/×10(6) sperm).

MAIN OUTCOME MEASURE(S):

Seminal ROS, TAC, sperm DNA fragmentation, ROS/TAC score were measured.

RESULT(S):

Group II had a high incidence of sperm DNA fragmentation than group I. The odds ratio of 1.25 for elevated ROS levels corresponded to >10% greater DNA fragmentation in our patients (95% confidence interval 1.01-1.53). Group II showed poor motility, a higher incidence of leukocytospermia, and higher ROS-TAC scores compared with group I. ROS was negatively correlated with sperm curvilinear velocity (r=-.24), linearity (r=-.24), and sperm motility (r=-.31). Sperm motility was correlated with %TUNEL(+ve) sperm (r=-.39).

CONCLUSION(S):

An increase in seminal ROS levels by 25% was associated with a 10% increase in sperm DNA fragmentation. Sperm motility was affected by seminal ROS and sperm DNA fragmentation.

[PubMed - indexed for MEDLINE]
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