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Diagn Microbiol Infect Dis. 2010 Feb;66(2):204-6. doi: 10.1016/j.diagmicrobio.2009.05.023.

Direct detection of Streptococcus pneumoniae in positive blood cultures by real-time polymerase chain reaction.

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Molecular Microbiology Department, University Hospital Sant Joan de Déu, Esplugues-Barcelona, Spain.


We developed a real-time polymerase chain reaction specific for Streptococcus pneumoniae to be applied directly from blood culture bottles without previous DNA extraction step. For the 128 blood culture bottles tested, the assay had 94% and 98.4% sensitivity and specificity, respectively. This assay provides rapid and accurate identification of this pathogen.

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