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Int J Mol Sci. 2009 Aug 4;10(8):3457-77. doi: 10.3390/ijms10083457.

Analysis of cooperativity by isothermal titration calorimetry.

Author information

1
Department of Biochemistry, University of Cambridge, UK. alan@cryst.bioc.cam.ac.uk

Abstract

Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples from the literature are used to demonstrate the applicability of ITC in the characterisation of cooperative systems.

KEYWORDS:

NMR; cooperativity; global analysis; isothermal titration calorimetry; multiprotein complexes; stoichiometry; thermodynamics

PMID:
20111687
PMCID:
PMC2812830
DOI:
10.3390/ijms10083457
[Indexed for MEDLINE]
Free PMC Article

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