Format

Send to

Choose Destination
Genome Biol. 2010 Jan 28;11(1):R9. doi: 10.1186/gb-2010-11-1-r9.

Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data.

Author information

1
Department of Pharmacological Sciences and Center for Stem Cell Research, University of Milan, 9 Via Balzaretti, Milan, 20133, Italy. manuela.marullo@unimi.it

Abstract

We describe a novel strategy for mRNA normalization in quantitative real-time PCR that is based on expressed Alu repeat amplification as a measure for the mRNA fraction. We show that expressed Alu repeat amplification is a fast, accurate normalization tool that can be successfully used for quantification of selected mRNA in the human transcriptome. This result is particularly important for clinical diagnosis and biomarker validation studies based on mRNA detection in human blood.

PMID:
20109193
PMCID:
PMC2847721
DOI:
10.1186/gb-2010-11-1-r9
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center