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PLoS One. 2010 Jan 20;5(1):e8763. doi: 10.1371/journal.pone.0008763.

Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.

Author information

1
Department of Biological Structure, University of Washington, Seattle, Washington, United States of America.

Abstract

BACKGROUND:

Inherited and acquired retinal degenerations are frequent causes of visual impairment and photoreceptor cell replacement therapy may restore visual function to these individuals. To provide a source of new retinal neurons for cell based therapies, we developed methods to derive retinal progenitors from human ES cells.

METHODOLOGY/PHYSICAL FINDINGS:

In this report we have used a similar method to direct induced pluripotent stem cells (iPS) from human fibroblasts to a retinal progenitor fate, competent to generate photoreceptors. We also found we could purify the photoreceptors derived from the iPS cells using fluorescence activated cell sorting (FACS) after labeling photoreceptors with a lentivirus driving GFP from the IRBP cis-regulatory sequences. Moreover, we found that when we transplanted the FACS purified iPSC derived photoreceptors, they were able to integrate into a normal mouse retina and express photoreceptor markers.

CONCLUSIONS:

This report provides evidence that enriched populations of human photoreceptors can be derived from iPS cells.

PMID:
20098701
PMCID:
PMC2808350
DOI:
10.1371/journal.pone.0008763
[Indexed for MEDLINE]
Free PMC Article
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