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Nat Struct Mol Biol. 2010 Feb;17(2):238-40. doi: 10.1038/nsmb.1768. Epub 2010 Jan 24.

Structural insights into the human GW182-PABC interaction in microRNA-mediated deadenylation.

Author information

1
Department of Molecular and Cell Biology, University of California, Berkeley, California, USA.

Abstract

GW182-family proteins are essential for microRNA-mediated translational repression and deadenylation in animal cells. Here we show that a conserved motif in the human GW182 paralog TNRC6C interacts with the C-terminal domain of polyadenylate binding protein 1 (PABC) and present the crystal structure of the complex. Mutations at the complex interface impair mRNA deadenylation in mammalian cell extracts, suggesting that the GW182-PABC interaction contributes to microRNA-mediated gene silencing.

PMID:
20098421
PMCID:
PMC2920127
DOI:
10.1038/nsmb.1768
[Indexed for MEDLINE]
Free PMC Article

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