Format

Send to

Choose Destination
See comment in PubMed Commons below
Bioelectrochemistry. 2010 Aug;79(1):95-100. doi: 10.1016/j.bioelechem.2009.12.007. Epub 2009 Dec 24.

Gadolinium blocks membrane permeabilization induced by nanosecond electric pulses and reduces cell death.

Author information

1
Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA, USA. franckandre1@gmail.com

Abstract

It has been widely accepted that nanosecond electric pulses (nsEP) are distinguished from micro- and millisecond duration pulses by their ability to cause intracellular effects and cell death with reduced effects on the cell plasma membrane. However, we found that nsEP-induced cell death is most likely mediated by the plasma membrane disruption. We showed that nsEP can cause long-lasting (minutes) increase in plasma membrane electrical conductance and disrupt electrolyte balance, followed by water uptake, cell swelling and blebbing. These effects of plasma membrane permeabilization could be blocked by Gd(3+) in a dose-dependent manner, with a threshold at sub-micromolar concentrations. Consequently, Gd(3+) protected cells from nsEP-induced cell death, thereby pointing to plasma membrane permeabilization as a likely primary mechanism of lethal cell damage.

PMID:
20097138
PMCID:
PMC2866748
DOI:
10.1016/j.bioelechem.2009.12.007
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center