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Curr Opin Plant Biol. 2010 Jun;13(3):280-87. doi: 10.1016/j.pbi.2009.12.008. Epub 2010 Jan 22.

Proteomics approaches to understand protein phosphorylation in pathway modulation.

Author information

1
Max Planck Institut für Molekulare Pflanzenpyhsiologie, Am Mühlenberg 1, 14476 Golm, Germany. wschulze@mpimpgolm.mpg.de

Abstract

Signaling pathways in all organisms consist of series of phosphorylation and dephosphorylation events that define directionality and allow different levels of feedback-regulation. Mass spectrometry-based proteomic analyses in recent years have led to a proteome-wide identification of thousands of phosphorylation sites in various plant species. Given this magnitude of mostly qualitative information about protein phosphorylation, discovery of specific phosphoproteins with regulatory functions represents a major challenge. In future large-scale experiments, combinations of data-driven modeling strategies based on quantitative data, targeted kinase-substrate screens, and verification in biochemical and genetic experiments are required to specifically spot phosphorylation sites with specific roles in signaling pathway modulation.

PMID:
20097120
DOI:
10.1016/j.pbi.2009.12.008
[Indexed for MEDLINE]

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