Abstract
To evaluate enzymes that produce hydrogen sulfide (H(2)S) in species of Fusobacterium nucleatum, we developed an assay based on SDS-polyacrylamide gel electrophoresis with renaturation followed by active staining. This assay provided precise insight into the enzymes that produce H(2)S in terms of their number and molecular weights.
Copyright 2010 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Electrophoresis, Polyacrylamide Gel / methods
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Fusobacterium Infections / complications
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Fusobacterium Infections / enzymology*
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Fusobacterium Infections / microbiology*
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Fusobacterium nucleatum / chemistry
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Fusobacterium nucleatum / metabolism*
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Humans
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Hydrogen Sulfide / metabolism*
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Molecular Weight
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Periodontal Diseases / enzymology
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Periodontal Diseases / etiology
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Species Specificity
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Staining and Labeling