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DNA Repair (Amst). 2010 Mar 2;9(3):210-23. doi: 10.1016/j.dnarep.2009.12.014. Epub 2010 Jan 25.

Is RecG a general guardian of the bacterial genome?

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1
Institute of Genetics, University of Nottingham, Queen's Medical Centre, Nottingham, NG7 2UH, United Kingdom.

Abstract

The RecG protein of Escherichia coli is a double-stranded DNA translocase that unwinds a variety of branched DNAs in vitro, including Holliday junctions, replication forks, D-loops and R-loops. Coupled with the reported pleiotropy of recG mutations, this broad range of potential targets has made it hard to pin down what the protein does in vivo, though roles in recombination and replication fork repair have been suggested. However, recent studies suggest that RecG provides a more general defence against pathological DNA replication. We have postulated that this is achieved through the ability of RecG to eliminate substrates that the replication restart protein, PriA, could otherwise exploit to re-replicate the chromosome. Without RecG, PriA triggers a cascade of events that interfere with the duplication and segregation of chromosomes. Here we review the studies that led us to this idea and to conclude that RecG may be both a specialist activity and a general guardian of the genome.

PMID:
20093100
DOI:
10.1016/j.dnarep.2009.12.014
[Indexed for MEDLINE]
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