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Cancer Lett. 2010 Jul 1;293(1):41-51. doi: 10.1016/j.canlet.2009.12.015. Epub 2010 Jan 21.

Genetic alterations in a telomerase-immortalized human esophageal epithelial cell line: implications for carcinogenesis.

Author information

1
Cancer Biology Group, Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

Abstract

Ectopic expression of viral oncoproteins disrupts cellular functions and limits the value of many existing immortalization models as models for carcinogenesis, especially for cancers without definitive viral etiology. Our newly established telomerase-immortalized human esophageal epithelial cell line, NE2-hTERT, retained nearly-diploid and non-tumorigenic characteristics, but exhibited genetic and genomic alterations commonly found in esophageal cancer, including progressive loss of the p16(INK4a) alleles, upregulation of anti-apoptotic proteins, epithelial-mesenchymal transition, whole-chromosome 7 gain and duplicated 5q arm. Our data also revealed a novel positive regulation of p16(INK4a) on cyclin D1. These findings probably represent early crucial events and mechanisms in esophageal carcinogenesis.

PMID:
20092939
DOI:
10.1016/j.canlet.2009.12.015
[Indexed for MEDLINE]
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