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J Microbiol Methods. 2010 Mar;80(3):310-2. doi: 10.1016/j.mimet.2010.01.001. Epub 2010 Jan 18.

Freezing of stool samples improves real-time PCR detection of Entamoeba dispar and Entamoeba histolytica.

Author information

1
Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Antwerp, Belgium. lcnops@itg.be

Abstract

PCR as a tool for intestinal parasite diagnosis is expanding since differentiation between Entamoeba histolytica and Entamoeba dispar cysts is impossible with microscopy. Since pre-analytical factors influence DNA detection, we evaluated with real-time PCR the influence of storage time and temperature. We demonstrated an improved DNA detection in frozen stool samples.

PMID:
20085792
DOI:
10.1016/j.mimet.2010.01.001
[Indexed for MEDLINE]

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