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Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1397-401. doi: 10.1073/pnas.0912182107. Epub 2010 Jan 4.

Platform for in situ real-time measurement of protein-induced conformational changes of DNA.

Author information

1
Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA.

Abstract

A platform for in situ and real-time measurement of protein-induced conformational changes in dsDNA is presented. We combine electrical orientation of surface-bound dsDNA probes with an optical technique to measure the kinetics of DNA conformational changes. The sequence-specific Escherichia coli integration host factor is utilized to demonstrate protein-induced bending upon binding of integration host factor to dsDNA probes. The effects of probe surface density on binding/bending kinetics are investigated. The platform can accommodate individual spots of microarrayed dsDNA on individually controlled, lithographically designed electrodes, making it amenable for use as a high throughput assay.

PMID:
20080702
PMCID:
PMC2824355
DOI:
10.1073/pnas.0912182107
[Indexed for MEDLINE]
Free PMC Article

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