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Proc Natl Acad Sci U S A. 2010 Jan 19;107(3):1053-8. doi: 10.1073/pnas.0913527107. Epub 2009 Dec 28.

Functional diversity among a family of human skeletal muscle myosin motors.

Author information

1
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, UCB347, Boulder, CO 80309, USA.

Erratum in

  • Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10765.

Abstract

Human skeletal muscle fibers express five highly conserved type-II myosin heavy chain (MyHC) genes in distinct spatial and temporal patterns. In addition, the human genome contains an intact sixth gene, MyHC-IIb, which is thought under most circumstances not to be expressed. The physiological and biochemical properties of individual muscle fibers correlate with the predominantly expressed MyHC isoform, but a functional analysis of homogenous skeletal muscle myosin isoforms has not been possible. This is due to the difficulties of separating the multiple isoforms usually coexpressed in muscle fibers, as well as the lack of an expression system that produces active recombinant type II skeletal muscle myosin. In this study we describe a mammalian muscle cell expression system and the functional analysis of all six recombinant human type II skeletal muscle myosin isoforms. The diverse biochemical activities and actin-filament velocities of these myosins indicate that they likely have distinct functions in muscle. Our data also show that ATPase activity and motility are generally correlated for human skeletal muscle myosins. The exception, MyHC-IIb, encodes a protein that is high in ATPase activity but slow in motility; this is the first functional analysis of the protein from this gene. In addition, the developmental isoforms, hypothesized to have low ATPase activity, were indistinguishable from adult-fast MyHC-IIa and the specialized MyHC-Extraocular isoform, that was predicted to be the fastest of all six isoforms but was functionally similar to the slower isoforms.

PMID:
20080549
PMCID:
PMC2824297
DOI:
10.1073/pnas.0913527107
[Indexed for MEDLINE]
Free PMC Article

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