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Nat Methods. 2010 Feb;7(2):133-6. doi: 10.1038/nmeth.1414. Epub 2010 Jan 10.

Genome-scale DNA methylation mapping of clinical samples at single-nucleotide resolution.

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1
Broad Institute, Cambridge, Massachusetts, USA.

Abstract

Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.

PMID:
20062050
PMCID:
PMC2860480
DOI:
10.1038/nmeth.1414
[Indexed for MEDLINE]
Free PMC Article
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