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Micron. 2010 Apr;41(3):257-62. doi: 10.1016/j.micron.2009.08.011. Epub 2009 Dec 3.

Connection between biomechanics and cytoskeleton structure of lymphocyte and Jurkat cells: An AFM study.

Author information

1
Chemistry Department, Jinan University, Guangzhou 510632, PR China.

Abstract

The mechanical properties of cells are important for many cellular processes. Here, atomic force microscopy (AFM) and laser scanning confocal microscopy (LSCM) were carried out to characterize lymphocyte and Jurkat cells. The average elastic modulus of lymphocyte is 1.24 +/- 0.09 kPa, which is almost twofold higher than that of Jurkat cell (0.51 +/- 0.06 kPa). LSCM images of sub-membrane cytoskeleton showed a significant difference in the organization of their F-actin structures. Lymphocyte cells had more and thicker actin bundles than that of Jurkat cells. Lymphocyte and Jurkat cells after adding the F-actin destabilizing agent Cytochalasin-B (Cyt-B) were also investigated by AFM. A decrease in the elastic modulus of lymphocyte from a value of 1.24 +/- 0.09 kPa down to 0.34 +/- 0.04 kPa for 24 h was observed, and that of Jurkat cell decreased from 0.51 +/- 0.06 kPa to 0.23 +/- 0.04 kPa. We really believe that this technology will be used for cancer detection and opens a door to study the biophysical properties of signaling domains extending from the cell surface to deeper parts of the cell.

PMID:
20060729
DOI:
10.1016/j.micron.2009.08.011
[Indexed for MEDLINE]

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