Format

Send to

Choose Destination
Mol Gen Genet. 1991 Feb;225(2):314-9.

Characterisation of the gene cysH and of its product phospho-adenylylsulphate reductase from Escherichia coli.

Author information

1
Biochemistry of Plants, Faculty of Biology, Ruhr University Bochum, Federal Republic of Germany.

Abstract

The nucleotide sequence of the gene cysH from Escherichia coli K12 was determined. The open reading frame was 735 nucleotides in length; it was flanked by a repetitive palindromic sequence centred 36 nucleotides upstream of cysH and a terminator-like structure located 20 nucleotides downstream. CysH encoded a polypeptide of Mr 27927 consisting of 244 amino acids. The gene product was isolated as a homodimer exhibiting phospho-adenylylsulphate reductase (PAPS reductase) activity. The active enzyme was devoid of electron transferring cofactors and contained only one cysteine per subunit. Reduction of the enzyme by dithiols resulted in a shift of the apparent molecular weight from 44,000 to 62,000 without formation of an enzyme-thioredoxin complex.

PMID:
2005873
DOI:
10.1007/bf00269864
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center