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J Clin Microbiol. 2010 Mar;48(3):697-702. doi: 10.1128/JCM.02021-09. Epub 2010 Jan 6.

Rapid identification and discrimination of Brucella isolates by use of real-time PCR and high-resolution melt analysis.

Author information

1
National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. jwinchell@cdc.gov

Abstract

Definitive identification of Brucella species remains a challenge due to the high degree of genetic homology shared within the genus. We report the development of a molecular technique which utilizes real-time PCR followed by high-resolution melt (HRM) curve analysis to reliably type members of this genus. Using a panel of seven primer sets, we tested 153 Brucella spp. isolates with >99% accuracy compared to traditional techniques. This assay provides a useful diagnostic tool that can rapidly type Brucella isolates and has the potential to detect novel species. This approach may also prove helpful for clinical, epidemiological and veterinary investigations.

PMID:
20053863
PMCID:
PMC2832424
DOI:
10.1128/JCM.02021-09
[Indexed for MEDLINE]
Free PMC Article

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