Format

Send to

Choose Destination
J Biochem Biophys Methods. 1991 Jan;22(1):19-22.

Microassay for DNA methyltransferase.

Author information

1
Department of Biochemistry, University of Glasgow, UK.

Abstract

A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37 degrees C with 3 microCi high specific activity [3H]AdoMet and 0.5 microgram poly[d(I-C).d(I-C)] in a volume of 23 microliters. Ribonuclease is present during the assay and the product DNA is isolated by phenol extraction after protease digestion.

PMID:
2005357
DOI:
10.1016/0165-022x(91)90077-a
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center