Format

Send to

Choose Destination
See comment in PubMed Commons below
J Gen Microbiol. 1993 Mar;139(3):465-72.

Positional distribution of fatty acids, and molecular species of polar lipids, in the diatom Phaeodactylum tricornutum.

Author information

1
Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1

Abstract

Each of the four main polar lipids from Phaeodactylum tricornutum UTEX 640, monogalactyosyldiacylglycerol(MGDG), digalactosyldiacylglycerol (DGDG), sulphoquinovosyldiacylglycerol (SQDG) and phosphatidylcholine(PC), was separated into its molecular species by reverse-phase HPLC, and the positional distribution of fatty acids in each species was determined. Numbers of separated peaks in each lipid class were: MGDG, 10; DGDG,7; SQDG, 10; and PC, 11. Eicosapentaenoic acid (EPA) was present in 20 of the 45 molecular species but predominated in the MGDG and DGDG classes, where 13 of the proposed structures contained EPA. EPA was always located in the sn-1 position except in two lipid species, MG5 and PC2, where it was present at both the sn-1 and sn-2 locations. The predominant polar lipid molecular species found in P. tricornutum UTEX 640 were(mg total fatty acids per g dry weight of biomass) 20:5-16:4-MG, 41-2; 20:5-16:1-DG, 21.0; 16:1-16:1-SQ,18-2; and 20:5-16: 1-MG, 18-0. [Structure indicates fatty acid at sn-1 position - fatty acid at sn-2 position -carbohydrate component at sn-3 position of the glycerol molecule.

ABBREVIATIONS:

20: 5, EPA; 16: 4,hexadecatetraenoic acid ; 16 : 1, palmitoleic acid ; MG, monogalactose ; DG, digalactose ; SQ, sulphoquinovose.]

PMID:
20050416
DOI:
10.1099/00221287-139-3-465
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center